Methods and compositions for enzyme complementation assays using the omega region of .beta.-galactosidase
US5362625A · kind A · utility
17Cited by
2References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 25, 1993 |
| Grant date | Nov 8, 1994 |
| Priority date | — |
| Expiry date | May 25, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/581
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the .beta.-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.