Replicative RNA reporter systems
US5364760A · kind A · utility
29Cited by
14References
53Claims
0Family size
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Key dates
| Filing date | Sep 22, 1992 |
| Grant date | Nov 15, 1994 |
| Priority date | — |
| Expiry date | Sep 22, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Highly sensitive methods for assaying for biopolymers, such as by immunoassay or nucleic acid probe hybridization assay, and compositions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q.beta.. The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.