Mutant microbial .alpha.-amylases with increased thermal, acid and/or alkaline stability
US5364782A · kind A · utility
8Cited by
2References
6Claims
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Key dates
| Filing date | Nov 29, 1990 |
| Grant date | Nov 15, 1994 |
| Priority date | — |
| Expiry date | Nov 29, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.