Patent · US Expired

Automated synthesis of oligonucleotides

US5368823A · kind A · utility

81Cited by
12References
26Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 11, 1993
Grant dateNov 29, 1994
Priority date
Expiry dateFeb 11, 2013

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC40B60/14
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

Apparatus and method for the automated synthesis of DNA segments utilizing multiple reaction columns, all of which are open at the inlet end to the atmosphere of a reaction chamber. A movable reagent supply line outlet is positioned adjacent to the column inlet end to apply reagent to each of the columns according to an input sequence of delivery. The delivery sequence is under processor control. Reagents are removed from all columns simultaneously through the application of vacuum at the outlet end of each column. The device enables the parallel synthesis of large numbers of different oligonucleotide sequences of different lengths.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.