Method of producing peptides

US5369018A · kind A · utility

Assignee

• Degussa AG · DE

Inventors

• Hans-Dieter Jakubke · Leipzig, DE
• Dirk Ullmann · Reutlingen, DE
• Karlheinz Drauz · Alzenau, DE
• Andreas Bommarius · Frankfurt, DE

Key dates

• Filing date: Apr 22, 1994
• Grant date: Nov 29, 1994
• Expiry date: Apr 22, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/023
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The biocatalytic production of a peptide is carried out with a zymogen instead of the customarily used protease. Since zymogens are proteolytically inactive precursors of proteases, proteolytic side reactions are largely suppressed. Preferably, amino acid esters or peptide esters and C-terminally derivatized amino acids or peptide fragments are coupled to each other. The reaction can also take place in a frozen, aqueous medium. In particular, chymotrypsinogen, trypsinogen, pepsinogen, prorennin or procarboxypeptidase are suitable as zymogens.