Isolated DNA encoding the NotI restriction endonuclease and related methods for producing the same
US5371006A · kind A · utility
2Cited by
1References
7Claims
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Key dates
| Filing date | Jan 11, 1993 |
| Grant date | Dec 6, 1994 |
| Priority date | — |
| Expiry date | Jan 11, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the NotI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia otitidis-caviarum into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity, and 3) purifying the NotI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.