Initial rate photometric method for immunoassay
US5371021A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 1992 |
| Grant date | Dec 6, 1994 |
| Priority date | — |
| Expiry date | Jul 17, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/272
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium's turbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including, haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.