Measurement of bacterial CO.sub.2 production in an isolated fluorophore by monitoring an absorbance regulated change of fluorescence
US5372784A · kind A · utility
15Cited by
22References
4Claims
0Family size
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Key dates
| Filing date | Mar 11, 1994 |
| Grant date | Dec 13, 1994 |
| Priority date | — |
| Expiry date | Mar 11, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multi-layer blood culture sensor includes two matrices. The first matrix is a polymer that is permeable to carbon dioxide and water, but impermeable to protons. A pH sensitive absorbance based dye is encapsulated or isolated in the polymer. The second matrix is a polymer with a pH insensitive fluorescent dye encapsulated or isolated in the polymer. The matrices are spectrally coupled and are useful for the determination of microorganisms in a blood culture bottle.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.