Immunoenzymatic single-plate ELISA method with competitive inhibition for detecting antisporozoite antibodies of plasmodium falciparum
US5374530A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 1992 |
| Grant date | Dec 20, 1994 |
| Priority date | — |
| Expiry date | May 13, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/967
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An ELISA method with competitive inhibition is described for determining antisporozoite antibodies of P. Falciparum in human blood samples and in mosquito extract, which uses a single plate pretreated with only the synthetic antigen (NANP).sub.20 using, as total competitive inhibitor for the formation of the complex between the synthetic antigen adsorbed on the plate and the antibody contained in the sample, the (NANP).sub.20 synthetic peptide in a weight ratio of at least 20:1 with respect to the adsorbed antigen. Because of its high specificity, sensitivity and speed, the method is particularly suitable for epidemiological studies on malaria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.