Test kit for determination of organophosphate and carbamate pesticides with insect brain material that hydrolyses a 6-substituted d luciferin ester
US5374535A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 23, 1993 |
| Grant date | Dec 20, 1994 |
| Priority date | — |
| Expiry date | Sep 23, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A test kit and a rapid and sensitive method are provided for the multiple detection of organophosphate and carbamate pesticides. The test method employs an insect brain material that hydrolyzes a 6-substituted D luciferin ester, preferably a 6-substituted acetyl D luciferin ester, to produce D luciferin. Hydrolysis is inhibited by the pesticides in a test sample. Luciferase and adenosine triphosphate react with the D luciferin to produce oxyluciferin and emitted bioluminescence. The brain material is initially incubated with a test sample followed by the addition of the ester, luciferase and adenosine triphosphate. The kit contains the brain material, the ester and optionally luciferase and adenosine triphosphate, and may also contain means for incubating, and means for measuring emitted bioluminescence, and a control standard.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.