Process for stabilizing 1-methylhydantoinase, use and method of it for determining an analyte and agents suitable for this

US5374546A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Rolf Nagel · Bürstadt, DE
• Ulfert Deneke · Fürth, DE
• Jurgen Mistele · Neulußheim, DE

Key dates

• Filing date: Jan 16, 1992
• Grant date: Dec 20, 1994
• Expiry date: Jan 16, 2012

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/986
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.