Bio-analytical separation method
US5375606A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1990 |
| Grant date | Dec 27, 1994 |
| Priority date | — |
| Expiry date | Feb 28, 2010 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K49/0091
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A separation method for use in conjunction with bio-analytical techniques, such as cell screening and blood flow measurement, in which a carrier bearing a bio-affecting compound comprising a reporter moiety is contacted with an organic extractant which is immiscible with the carrier, so as to produce distinct extractant and carrier phases, with a major amount of the bio-affecting compound originally associated with the carrier being transferred to the extractant phase. The separation method is particularly useful in performing bio-analytical techniques involving fluorometric determinations, as fluorescence efficiency is increased substantially by transfer of a fluorescent reporter from an aqueous carrier phase to the organic extractant phase, and concentration of the fluorescent reporter in the extractant phase prior to fluorescence measurement.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.