Method for determining the biodistribution of substances using fluorescence spectroscopy
US5377676A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 30, 1993 |
| Grant date | Jan 3, 1995 |
| Priority date | — |
| Expiry date | Mar 30, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61B2090/3941
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for determining the biodistribution of substances using fluorescence spectroscopy. A photosensitizing agent or other intrinsically fluorescent agent, or an agent labeled with an extrinsic fluorophor is administered to a subject. A fiberoptic probe integrated with an excitation light source illuminates the examined tissue and causes fluorescence. The fluorescence is recorded by a spectrograph and plotted as a spectral curve. The intensity ratio (S1/S2) for the fluorescence from the photosensitizing agent (S1) and autofluorescence (S2) for the examined tissue is used as an index for drug presence and compared with the intensity ratio at the same wavelengths for various tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.