Method for studying nucleic acids within immobilized specimens
US5382511A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 24, 1992 |
| Grant date | Jan 17, 1995 |
| Priority date | — |
| Expiry date | Aug 24, 2012 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0406
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for the automated detection of target nucleic acid sequences in which multiple biological samples are individually incorporated into a matrix and treated in a stepwise fashion to expose, amplify, detect and enumerate the presence of original, target nucleotide sequences in each sample. The samples are held together with other samples in a chamber connected by means of a fluid flow conduit to which the matrices make connection via numerous jet-spray ports for the injection of various fluids from a plurality of reservoirs used to process the samples. Solvents and reagents are used to degrade and wash cellular structures and contaminants away from the nucleic acids. Primer and polymerase molecules are used to replicate the target sequences of nucleic acids in the sample. Labeled DNA probe molecules serve to hybridize to the amplified target sequences for detection. Appropriate buffers allow these manipulations on the nucleic acid molecules in the samples. The system also includes a pump to draw the fluids from the reservoirs and force them through the jet spray manifolds into the main chamber and valves under the control of a microprocessor. The system also includes fans …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.