Process for the purification of cyclosporin A
US5382655A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 25, 1993 |
| Grant date | Jan 17, 1995 |
| Priority date | — |
| Expiry date | May 25, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/645
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is disclosed for the solution chromatographic purification of cyclosporin A from a starting mixture containing one or more of cyclosporin A, B, C, other cyclosporin components that are more polar or more apolar than cyclosporin A, and other like contaminants, by heating the starting mixture or an evaporation residue thereof to a temperature from about 80.degree. C. to about 115.degree. C., melting the heated starting mixture, and carrying out solution chromatography of the melted material, suitably first in a 48:50:2 mixture of chloroform, dichloromethane, and ethanol, and then in a mixture of like proportions, of the solvents chloroform, ethylacetate, and ethanol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.