Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5384253A · kind A · utility
322Cited by
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21Claims
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Key dates
| Filing date | Dec 28, 1990 |
| Grant date | Jan 24, 1995 |
| Priority date | — |
| Expiry date | Dec 28, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8206
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.