Glucose isomerases with an altered pH optimum
US5384257A · kind A · utility
6Cited by
3References
15Claims
0Family size
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Key dates
| Filing date | Aug 26, 1993 |
| Grant date | Jan 24, 1995 |
| Priority date | — |
| Expiry date | Aug 26, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.