Nonwoven fabric coated with a mixture of silk fibroin, gelatin and insolubilized chitosan for use as a carrier for animal cells
US5385836A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | May 18, 1992 |
| Grant date | Jan 31, 1995 |
| Priority date | — |
| Expiry date | May 18, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/72
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A carrier for adhering animal cells during culturing or for immobilization of animal cells is produced by coating a porous substrate with a cell adhesive material in the form of a mixture with chitosan. In a preferred embodiment, the porous substrate is a nonwoven fabric prepared by impregnating a nonwoven fabric web with a binder resin, and the mixture contains silk fibroin, gelatin and chitosan. Coating is carried out by contacting the nonwoven fabric with a solution prepared by adding silk fibroin and gelatin to an acidic aqueous solution of chitosan to coat the nonwoven fabric, drying the coated nonwoven fabric and treating the dried nonwoven fabric with an alkali to render the chitosan insoluble.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.