Method for specifically altering the nucleotide sequence of RNA
US5389514A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 28, 1992 |
| Grant date | Feb 14, 1995 |
| Priority date | — |
| Expiry date | Aug 28, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/10122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is provided for specifically altering the nucleotide sequence of an RNA molecule. As performed in vitro the method comprises: (1) selecting a target sequence in an RNA molecule; (3) hybridizing the target sequence with a complementary nucleic acid, either DNA or RNA, thereby forming a double-stranded structure; (4) incubating the hybrid molecule with a cellular extract which contains components capable of specifically converting U to C in an RNA molecule, when the U is disposed within a double-stranded structure. Incubation of the hybrid molecule with cellular extracts comprising the RNA editing component results in a specific alteration of the nucleotide sequence of the RNA at the target sequence. The method of the invention may also be applied in vivo. Complementary nucleic acids are introduced into cells and hybridized to the target sequence to form a double-stranded structure. The RNA editing components already present in the cell may then specifically convert U to C in the target sequence resulting in an alteration in the target RNA molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.