Plasmid vectors for cellular slime moulds of the genus dictyostelium
US5389526A · kind A · utility
Inventors
Key dates
| Filing date | Jun 25, 1992 |
| Grant date | Feb 14, 1995 |
| Priority date | — |
| Expiry date | Jun 25, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.