Electrochemical immunoassay methods
US5391272A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 8, 1994 |
| Grant date | Feb 21, 1995 |
| Priority date | — |
| Expiry date | Mar 8, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electro-immunosensor which can be used to determine the presence of a wide range of analytes in solution is described. Also described are methods of determining subnanogram levels of analytes in a one-step procedure. This novel immunosensor comprises an analyte binding agent bound to an electrode and a analyte/enzyme conjugate bound to the analyte binding agent as part of a catalytic electrical circuit. Displacement of the conjugate by analyte causes a proportional decrease in current. The immunosensor may also be adapted to detect two analytes in a single step by using different enzymes conjugated to the analyte binding agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.