Method for detecting a nucleotide at a specific location within a nucleic acid using exonuclease activity
US5391480A · kind A · utility
84Cited by
2References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 3, 1991 |
| Grant date | Feb 21, 1995 |
| Priority date | — |
| Expiry date | Dec 3, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence or absence of the test nucleotide then may be established by determining whether the label is preserved or lost following the reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.