Reagent for Determining .alpha.-amylase activity and method for determining .alpha.-amylase activity
US5393660A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 4, 1993 |
| Grant date | Feb 28, 1995 |
| Priority date | — |
| Expiry date | Nov 4, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2334/10
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.