Blocked lectins; methods and affinity support for making the same using affinity ligands; and method of killing selected cell populations having reduced non-selective cytotoxicity
US5395924A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 19, 1993 |
| Grant date | Mar 7, 1995 |
| Priority date | — |
| Expiry date | Feb 19, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K47/6817
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An activated affinity ligand is described comprising: a ligand having (a) a region with affinity for binding sites of a lectin; and (b) a reactive group capable of covalently linking the ligand to the lectin to thereby block one or more of the binding sites of the lectin. A blocked lectin is described comprising one or more affinity ligands covalently linked by means of a reactive group present on each of the ligands to a lectin such that one or more binding sites of the lectin is blocked. A cell-binding agent-blocked lectin conjugate is described comprising the above-described blocked lectin and a cell-binding agent covalently linked to: (a) one of the covalently linked affinity ligands; or (b) the lectin. A method of preparing the cell-binding agent-blocked lectin conjugate is described. An affinity support capable of binding to a lectin to form a blocked lectin is described comprising an activated affinity ligand covalently linked to a solid support. A method of preparing the affinity support capable of binding to a lectin to form a blocked lectin is described. A method of killing selected cell populations having reduced cytotoxicity to non-selected cell populations is described…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.