Attachment of compounds to polymeric particles using carbamoylonium compounds and a kit containing same
US5397695A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 29, 1989 |
| Grant date | Mar 14, 1995 |
| Priority date | — |
| Expiry date | Jun 29, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, such as enzymes, polypeptides and proteins. This attachment is carried out using carbamoylonium compounds which react with the carboxyl groups to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a carbamoylonium compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.