Thermally stable and pH stable subtilisin analogs and method for production thereof
US5399283A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 1991 |
| Grant date | Mar 21, 1995 |
| Priority date | — |
| Expiry date | Jan 9, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A mutated subtilisin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of asparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Asn or a Gly in an Asn-Gly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.