Nucleic acid sequence amplification methods
US5399491A · kind A · utility
869Cited by
2References
49Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 19, 1992 |
| Grant date | Mar 21, 1995 |
| Priority date | — |
| Expiry date | Mar 19, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.