Method for labeling the 3' terminus of a synthetic oligonucleotide using a unique multifunctional controlled pore glass (MF-CPG) reagent in solid phase oligonucleotide synthesis
US5401837A · kind A · utility
26Cited by
2References
9Claims
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Key dates
| Filing date | Aug 24, 1992 |
| Grant date | Mar 28, 1995 |
| Priority date | — |
| Expiry date | Aug 24, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2982
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for derivatizing and labeling the 3'-terminus of an oligonucleotide during solid phase synthesis comprising the use of a multifunctional reagent whose preferred structure is shown below. ##STR1## wherein CPG is controlled pore glass beads, Fmoc is 9-fluorenylmethyoxycarbonyl, and the alkylamine contains 1 to 50 carbon atoms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.