Patent · US Expired

Precipitate test for microorganisms

US5403721A · kind A · utility

46Cited by
15References
15Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 21, 1993
Grant dateApr 4, 1995
Priority date
Expiry dateApr 21, 2013

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/97
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

There is disclosed an improved method for testing for the presence of a particular microorganism or group of microorganisms characterized by a particular enzyme. The method uses a dye-forming substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate when cleaved by the enzyme. The precipitate is concentrated to the polymer matrix and/or solid support member to create a visible reaction product, wherein the amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming substrate throughout a polymer system or on the surface of a solid support member.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.