Method of sequencing DNA
US5405746A · kind A · utility
111Cited by
2References
13Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 22, 1993 |
| Grant date | Apr 11, 1995 |
| Priority date | — |
| Expiry date | Sep 22, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method of sequencing target DNA in which said DNA is provided in double stranded form immobilized on a solid support via one terminus of one of the two strands thereof and is then subjected to strand separation whereby the unattached strand is removed prior to sequencing the immobilized strand. The target DNA, e.g genomic DNA, may initially be amplified by the PCR method using at least one oligonucleotide primer which is either attached to a solid support or carries means for attachment to a solid support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.