Sequence-specific binding polymers for duplex nucleic acids
US5405938A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 23, 1992 |
| Grant date | Apr 11, 1995 |
| Priority date | — |
| Expiry date | Nov 23, 2012 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/0072
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention describes a polymer composition effective to bind, in a sequence-specific manner to a target sequence of a duplex polynucleotide, at least two different-oriented Watson/Crick base-pairs at selected positions in the target sequence. The composition includes an uncharged backbone with 5- or 6-membered cyclic backbone structures and selected bases attached to the backbone structures effective to hydrogen bond specifically with different oriented base-pairs in the target sequence. Also disclosed are subunits useful for the construction of the polymer composition. The present invention further includes methods for (i) coupling a first free or polymer-terminal subunit, and (ii) isolating, from a liquid sample, a target duplex nucleic acid fragment having a selected sequence of base-pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.