Method and system for determining bioactive substances
US5411866A · kind A · utility
454Cited by
7References
7Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 30, 1993 |
| Grant date | May 2, 1995 |
| Priority date | — |
| Expiry date | Mar 30, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6812
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic acid. Prior to enzymatic degradation and amperometric detection of the products or elements resulting from the degradation, the interfering substances are retained by an anion exchanger disposed upstream of the enzymatic degradation site. The conditions of the method are controlled to utilize the difference of isoelectric points of the measured biosubstance and of the interfering compounds respectively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.