Erythrocyte agglutination assay

US5413913A · kind A · utility

Assignee

• Agen Biomedical Limited · AU

Inventors

• Carmel J. Hillyard · Forsayth, AU
• Dennis Rylatt · Melbourne, AU
• Bruce E. Kemp · Melbourne, AU
• Peter G. Bundesen · Brisbane, AU

Key dates

• Filing date: Feb 3, 1994
• Grant date: May 9, 1995
• Expiry date: Feb 3, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/866
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte (in the case of a direct assay) or analyte binding reagent (in the case of an indirect assay). Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed.