Homologous recombination for universal donor cells and chimeric mammalian hosts

US5413923A · kind A · utility

Assignees

• Cell Genesys, Inc. · US
• Univ. of North Carolina at Chapel Hill · US

Inventors

• Raju Kucherlapati · Darien, US
• Beverly Koller · Carrboro, US
• Oliver Smithies · Durham, US

Key dates

• Filing date: Dec 11, 1992
• Grant date: May 9, 1995
• Expiry date: Dec 11, 2012

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01K2267/03
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies.