Reagent for measuring immature leukocytes
US5413938A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 17, 1994 |
| Grant date | May 9, 1995 |
| Priority date | — |
| Expiry date | Mar 17, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2525
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A reagent for measuring immature leukocytes which comprises: (1) a polyoxyethylene-based nonionic surfactant having the general formula (I) in a sufficient amount capable of fixing cytoplasms and cell membranes of immature leukocytes: EQU R.sub.1 --R.sub.2 --(CH.sub.2 CH.sub.2 O ).sub.n --H (I) where R.sub.1 is an alkyl, alkenyl or alkynyl group having 10 to 25 carbon atoms; R.sub.2 is --O--, --(C.sub.6 H.sub.4)--O-- or --COO--; and n is an integer of 10 to 40, (2) a solubilizing agent in a sufficient amount capable of damaging cell membranes of blood cells other than immature leukocytes and shrinking them, (3) an amino acid in a sufficient amount capable of stabilizing cytoplasm and cell membrane of immature leukocytes, and (4) an aqueous medium, which adjusts pH value in the range of 5.0 to 9.0, osmolarity in the range of 150 to 600 mOsm/kg and electric conductivity in the range of 6.0 to 9.0 mS/cm, respectively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.