Apparatus and method for amplifying and detecting target nucleic acids
US5415839A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 1993 |
| Grant date | May 16, 1995 |
| Priority date | — |
| Expiry date | Oct 21, 2013 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L7/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.