Fluorescent lipid polymer-macromolecular ligand compositions as detection element in ligand assays
US5415999A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 1993 |
| Grant date | May 16, 1995 |
| Priority date | — |
| Expiry date | Jul 9, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions are provided for the detection of analytes. The method employs a fluorescence production layer which comprises a fluorescent polymerized polyunsaturated lipid layer in association with a ligand which is a member of a specific binding pair, where the ligand is competitive with the analyte for the complementary binding member or is a complementary binding member. By providing for a fluorescence modulation reagent which binds to the fluorescence production layer in proportion to the amount of analyte in the sample, by measuring the resulting fluorescence after carrying out the assay methodology, the amount of analyte can be determined quantitatively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.