Cross-regulation of gene expression in recombinant cells
US5416008A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 1, 1991 |
| Grant date | May 16, 1995 |
| Priority date | — |
| Expiry date | Oct 1, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant cells providing for the controlled expression of product proteins by way of cross-regulation between interacting operons. A structural gene for a product protein and a structural gene for a repressor of a second operon are included in a first operon. A protein encoded by a structural gene of the second operon is a repressor of the first operon. The second operon may reside on a plasmid or a chromosome of the host cell. The present invention provides for controlled expression of product protein over a range of copy numbers, as well as high transcription efficiency in the induced state. The invention includes methods for the controlled expression of product protein by recombinant cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.