Patent · US Expired

Cloned N-carbamoyl sarcosine amidohydrolase

US5416014A · kind A · utility

3Cited by

5References

17Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Helmut Burtscher · Habach, DE
Gunther Schumacher · Bernried, DE

Key dates

Filing date	Aug 17, 1993
Grant date	May 16, 1995
Priority date	—
Expiry date	Aug 17, 2013

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/849
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns a recombinant DNA which contains PA1 (1) the sequence shown in SEQ ID NO: 1, PA1 (2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or PA1 (3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent hybridizing conditions whereby the DNA sequence codes for a protein with N-carbamoyl sarcosine amidohydrolase activity. Furthermore the invention concerns a recombinant vector with the DNA according to the present invention as well as a process for the isolation of a recombinant protein with N-carbamoyl sarcosine amidohydrolase activity and its use for the determination of creatinine.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.