Quantitative measurement of LDL

US5417863A · kind A · utility

Assignee

• PerSeptive Biosystems Inc. · US

Inventors

• Laszlo Varady · Malden, US
• Noubar B. Afeyan · Lexington, US
• Robert S. Langer · Newton, US
• Shmuel David Shefer · East Brunswick Township, US

Key dates

• Filing date: Jun 30, 1994
• Grant date: May 23, 1995
• Expiry date: Jun 30, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed is a method for the rapid quantitative determination of low density lipoprotein (LDL) in a biological fluid sample containing LDL, very low density lipoprotein (VLDL) and high density lipoprotein (HDL). The sample is first passed through a matrix to bind LDL to the matrix. The LDL is then eluted from the matrix differentially from VLDL and HDL. A parameter representative of the concentration of a solute exiting the matrix during the interval when LDL is eluted is then determined, wherein the parameter is proportional to the amount of the LDL in the sample. The parameter may be determined, e.g., by electronically integrating under a spectrophotometrically detected peak of eluted LDL.