Liposomal antisense methyl phosphonate oligonucleotides and methods for their preparation and use
US5417978A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 29, 1993 |
| Grant date | May 23, 1995 |
| Priority date | — |
| Expiry date | Jul 29, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3125
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A liposomal methyl phosphonate oligonucleotide composition useful in treatment of chronic myeloid leukemia comprises (a) a liposome which comprises at least one phospholipid, and (b) an antisense methyl phosphonate oligonucleotide which is entrapped in the liposome. The molar ratio of phospholipid in the liposome to the methyl phosphonate entrapped in the liposome is between about 100:1 and about 10,000:1. A process for making the composition includes the steps of (a) mixing an antisense methyl phosphonate oligonucleotide in a first organic solvent with at least one phospholipid in a second organic solvent, where the molar ratio of phospholipid to methyl phosphonate is between about 100:1 and about 10,000:1, (b) lyophilizing the mixture formed in step (a), producing a lyophilized powder, (c) hydrating the lyophilized powder, and (d) sonicating the hydrated material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.