Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5420032A · kind A · utility
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11Claims
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Key dates
| Filing date | Dec 16, 1992 |
| Grant date | May 30, 1995 |
| Priority date | — |
| Expiry date | Dec 16, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a homing endonuclease which originates from Chlamydomonas eugametos, and was overproduced in E. coli, purified and characterized. The homing endonuclease of the present invention recognizes and cleaves degenerate double-stranded DNA at a specific recognition site; it particularly recognizes and cleaves 15, 17 and 19 nucleotide sequences. The cleavage of target DNA by this endonuclease produces a 4 nucleotide extension with a 3' OH overhang. A method to use the endonuclease of the present invention to cleave DNA fragments useful for gene mapping is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.