Method for the analytical determination of the concentration of a component of a medical sample

US5420042A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Rainer Schafer · Schneeberg, DE
• Christoph Berding · Hersbruck, DE
• Fridl Lang · Tutzing, DE
• Wilheim Kleider · Rosenheim, DE
• Peter Wolf · Wiesbaden, DE

Key dates

• Filing date: Jun 29, 1993
• Grant date: May 30, 1995
• Expiry date: Jun 29, 2013

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/11
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method for the analytical determination of the concentration of a component of a medical sample, in which a reaction of the sample with reagents leads to a time-dependent change S(t) in a measured quantity S and the concentration C correlates according to an evaluation curve C(X) with an input variable X derived from S(t), in which the calibration curve is ambiguous for at least a portion of the possible X values. In order to assign an input variable X to one of the sub-sections and thereby to obtain an unambiguous correlation to a particular concentration C, a training run and an analysis run are performed. In the training run, a discrimination algorithm is performed at least once, in which a discriminator set is generated from measurements of S(t), a score is generated in each case from the latter with a multivariate statistical technique and it is checked whether the scores can be divided into separate subsets, in which the concentrations are correctly assigned to the sub-sections of the calibration curve. The discrimination algorithm is repeated with different discriminator sets generated according to different generation methods from the measurements of S(t), in order to estab…