Separation of phosphorothioate oligonucleotides by capillary gel electrophoresis
US5420265A · kind A · utility
1Cited by
7References
12Claims
0Family size
Assignee
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Key dates
| Filing date | Dec 16, 1992 |
| Grant date | May 30, 1995 |
| Priority date | — |
| Expiry date | Dec 16, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for separating a mixture of 5'-phosphorothioate mononucleotides, a mixture of 5'-phosphorothioate oligonucleotides, or a mixture of both 5'-phosphorothioate mononucleotides and 5'-phosphorothioate oligonucleotides comprising electro- phoresis through a gel of at least 12% polymerized linear acrylamide containing at least 50% formamide or other denaturing organic solvent. This method can resolve phosphorothioate oligonucleotides differing by only a single base. Samples of less than 1 nanogram in microliter volumes can be conveniently handled.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.