Methods for the recovery of nucleic acids from reaction mixtures
US5422241A · kind A · utility
62Cited by
1References
13Claims
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Key dates
| Filing date | Jul 3, 1991 |
| Grant date | Jun 6, 1995 |
| Priority date | — |
| Expiry date | Jul 3, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the recovery of nucleic acids from a reaction mixture, such as nuclease protection assays, comprising the use of one reagent containing a) nuclease for digesting single-stranded RNA and b) a nucleic acid precipitating carrier agent, (sheared DNA or linear acrylamide). A second reagent contains a chaotropic agent (a guanidinium salt) for inactivating said nucleases and an alcohol (ethanol or isopropanol) for the simultaneous inactivation of the nucleases and the precipitation of the nucleic acids without the need for protease digestion or organic extraction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.