Bidentate conjugate and method of use thereof
US5422281A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1992 |
| Grant date | Jun 6, 1995 |
| Priority date | — |
| Expiry date | Jul 31, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/822
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A novel bidentate conjugate has two different chemical moieties, or bidentate members, attached through an adequate spacer moiety. Each bidentate member acts as a small molecule ligand and is capable of specifically binding to a different macromolecular substance. The bidentate members are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected so that both bidentate members can simultaneously bind to their respective specific binding partners. Where the specific binding partners are multivalent, large complexes can be formed. The formation of these complexes can be inhibited by the presence of an unconjugated monovalent bidentate member, such as free analyte from a test sample. The bidentate is of particular use in turbidimetric or nephelometric inhibition immunoassay procedures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.