Antibiotic cryptdin peptides and methods of their use

US5422424A · kind A · utility

Assignees

• The Regents of the University of California · US
• The Shriner's Hospital For Crippled Children · US
• The General Hospital Corporation · US

Inventors

• Michael E. Selsted · Los Angeles, US
• Andre J. Ouellette · Lynn, US
• Samuel I. Miller · Brookline, US

Key dates

• Filing date: Aug 14, 1992
• Grant date: Jun 6, 1995
• Expiry date: Aug 14, 2012

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/806
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence as follows: PA1 X.sub.1 -C-X.sub.2 -C-R-X.sub.3 -C-X.sub.4 -E-X.sub.5 -G-X.sub.6 -C-X.sub.7 -C-C-X.sub.8 wherein X.sub.1 is 3-6 amino acids; X.sub.2 is one amino acid; X.sub.3 is 2 or 3 amino acids; X.sub.4 is three amino acids; X.sub.5 is three amino acids; X.sub.6 is one amino acid; X.sub.7 is 6 to 10 amino acids; and X.sub.8 is 0 to 7 amino acids. The invention further provides substantially purified cryptdin peptides having a consensus amino acid sequence as follows: PA1 X.sub.1 -L-X.sub.2 -C-Y-C-R-X.sub.3 -C-K-X.sub.4 -E-R-X.sub.5 -G-T-C-X.sub.6 -C-C-X.sub.7 wherein X.sub.1 is one to four amino acids; X.sub.2 is one amino acid; X.sub.3 is three amino acids; X.sub.4 is two amino acids; X.sub.5 is two amino acids; and X.sub.6 is six to nine amino acids; and X.sub.7 is zero to seven amino acids. The illustrated embodiments have amino acids selected from the group consisting of the following sequences: PA1 LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR PA1 LRDLVCYCRTRGCKRRERMNGTCRKGHLMYTLCCR PA1 LRDLVCYCRKRGCKRRERMNGTCRKGHLMYTLCCR PA1 GLLCYCRKGHCKRGERVRGTCGIRFLYCCPR PA1 LSKKLICYCRIR…