DNA sequence-based HLA class I typing method
US5424184A · kind A · utility
21Cited by
4References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 8, 1991 |
| Grant date | Jun 13, 1995 |
| Priority date | — |
| Expiry date | May 8, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/172
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.