Amplified hybridization assay
US5424188A · kind A · utility
37Cited by
15References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1992 |
| Grant date | Jun 13, 1995 |
| Priority date | — |
| Expiry date | Oct 20, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A kit for an amplified hybridization assay is described in which a family of signal-generating secondary probes bind to a primary probe that hybridizes to the target sequence of interest. Thus, an enormously amplified signal is generated by the hybridization event. The assay can be used for a variety of laboratory and clinical purposes and is automatable. A hybridization assay kit is also described. The kit is used for the detection of a target nucleotide sequence. One embodiment of the kit includes a plurality of secondary probes, each secondary probe capable of binding to a distinct binding site of the primary probe.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.