Patent · US Expired

Amyloidin protease and uses thereof

US5424205A · kind A · utility

41Cited by
3References
11Claims
0Family size

Assignees

Inventors

Key dates

Filing dateMay 7, 1993
Grant dateJun 13, 1995
Priority date
Expiry dateMay 7, 2013

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N9/6421
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A proteolytic enzyme isolated from human tissue which exhibits a proteolytic activity to hydrolyze Met-Asp peptide bond in an amyloid-like substrate is disclosed. This enzyme has been designated "amyloidin" because it proteolytically cleaves a Met-Asp bond similar to the one present in the amyloid precursor protein to release a fragment having the mature Asp terminus of the .beta.-amyloid peptide. Antibodies to the amyloidin protease are also provided. Methods to isolate and purify the amyloidin protease are provided, as well as assays to screen for inhibitors of the amyloidin protease. Also disclosed is the gene encoding the protease and methods for expression of the protease by recombinant DNA means.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.